Equipment
The Immune Modeling, Analysis and Diagnostics Shared Resource has the following equipment and infrastructure available:
- Bio-Plex 200 system capable of multiplexing up to 100 analytes simultaneously in a single well using only 25 ul of sample
- Tissue culture room for patient sample processing and to perform immune function assays. Equipment includes 3 laminar hoods, 4 tissue culture incubators, centrifuges (two Eppendorf 5425R and 5810R), microscope and water bath
- Infrastructure for sample collection, processing and storage with dedicated -20C and -80C freezers and two large liquid nitrogen storage units with temperature monitors
- QiaCube robot for automated nucleic acid isolation
- Eppendorf epMotion® 5075t
- Agilent 2200 TapeStation to check RNA/DNA/protein integrity
- Bio-Rad Droplet Digital PCR system
- Tecan Infinite M1000 PRO microplate reader capable of 3D imaging and top and bottom reads
- Nikon Eclipse TS100 fluorescence microscope and camera
- 3 PCR cabinet workstations
- Bio-Rad CFX96 quantitative real-time PCR system
- Five Bio-Rad 96-well PCR machines
- Qiagen TissueLyser II
- Qubit Fluorometer
- Three Illumina high-speed shakers with thermo capabilities
- MiSeq next generation sequencer
- 10X Genomics Chromium X Series Controller