User Information - Genomics
The Genomics Shared Resource, or GSR, provides centralized, cost-effective, and comprehensive expertise and service in the area of next-generation sequencing-based genomics research.
Protocols
RNA isolation:
These are the recommended protocols for isolation of high-quality RNA for use in RNA-Seq and small RNA-Seq library preparations. The GSR does not recommend using other protocols to isolate RNA for submission to the GSR.
Useful RNA-related links:
Below are a number of useful online resources related to working with RNA. To submit additional helpful links for inclusion below, please contact the GSR.
- Practical Tips for Handling RNA
- Working with RNA
- How to Maintain an RNase-free Lab
- Methods to Check RNA Integrity
- Assessing RNA Quality Movie
DNA isolation: (Under construction)
These are the recommended protocols for isolation of high-quality genomic DNA for use in whole-genome and whole-exome sequencing analyses.